 Dewar Challenge Cup | |
| Tournament details | |
|---|---|
| Country |  USA |
| Defending champions | Los Angeles Kickers |
| Final positions | |
| Champions | New York Ukrainians (1st title) |
| Runner-up | Chicago Hansa |
The 1965 National Challenge Cup was the 52nd edition of the USSFA's annual open soccer championship. The New York Ukrainians defeated the Chicago Hansa to win.