The 2000 Exxon Superflo 12 Hours of Sebring was the 48th running of the 12 Hours of Sebring as well as the opening round of the 2000 American Le Mans Series season. It took place at Sebring International Raceway, Florida, on March 18, 2000. This was the first time ever the 12 Hours was covered flag-to-flag on television with Speedvision doing the honors.