| Duration | 4 rounds |
|---|---|
| Number of teams | 13 |
| Winners | Thatto Heath St Helens |
| Runners-up | Leigh Miners Rangers |
| Biggest home win | Leigh Miners Rangers 58 – 0 Brighouse Rangers |
| Biggest away win | Normanton Knights 4 – 62 Thatto Heath St Helens |
The 2016 RFL Women's Challenge Cup was an English rugby league knockout tournament competed for by 12 teams during the summer of 2016. The Challenge Cup was won by Thatto Heath St Helens who beat Leigh Miners Rangers 6–62 in the final. The Challenge Shield was won by the Thatto Heath Reserves team who beat Whitley Bay Barbarians 0–44 in the final. Both finals took place on 31 July 2016 at the Provident Stadium, Bradford.