| 2021 Challenger Ciudad de Guayaquil | |
|---|---|
| Date | 1–7 November |
| Edition | 17th |
| Surface | Clay |
| Location | Guayaquil, Ecuador |
| Champions | |
| Singles | |
 Alejandro Tabilo | |
| Doubles | |
 Jesper de Jong / Bart Stevens | |
The 2021 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the seventeenth edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 1 and 7 November 2021.