3T3-L1

Differentiated adipocytes in a 3T3-L1 cell line stained with Oil Red O

3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions. The 3T3-L1 Swiss subclone line has been widely utilized, since its development, due to its affinity for lipid droplet deposition in vitro. 3T3-L1 cells have a fibroblast-like morphology, but, under appropriate conditions, the cells differentiate into an adipocyte-like phenotype, providing an exemplar model for white adipocytes.[1][2][3] 3T3-L1 cells can be utilized to study a number of cellular and molecular mechanisms related to insulin-resistance, obesity, and diabetes in vitro.[2] Aside from its usages, this cell line is widely developed and can be purchased for continuous propagation for numerous research studies.[4] 3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring appearance of adipose cells. These cells are also sensitive to lipogenic and lipolytic hormones, as well as drugs, including epinephrine, isoproterenol, and insulin.[5]

  1. ^ "3T3-L1 Cell Line". Retrieved 22 July 2018.
  2. ^ a b Green, Howard; Kehinde, Olaniyi (March 1974). "Sublines of mouse 3T3 cells that accumulate lipid". Cell. 1 (3): 113–116. doi:10.1016/0092-8674(74)90126-3. ISSN 0092-8674.
  3. ^ Zebisch, Katja; Voigt, Valerie; Wabitsch, Martin; Brandsch, Matthias (June 2012). "Protocol for effective differentiation of 3T3-L1 cells to adipocytes". Analytical Biochemistry. 425 (1): 88–90. doi:10.1016/j.ab.2012.03.005. ISSN 0003-2697. PMID 22425542.
  4. ^ Poulos, Sylvia P; Dodson, Michael V; Hausman, Gary J (October 2010). "Cell line models for differentiation: preadipocytes and adipocytes". Experimental Biology and Medicine. 235 (10): 1185–1193. doi:10.1258/ebm.2010.010063. ISSN 1535-3702. PMID 20864461. S2CID 24179092.
  5. ^ Green H, Kehinde O (1975). "An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion". Cell. 5 (1): 19–27. doi:10.1016/0092-8674(75)90087-2. PMID 165899. S2CID 19040294.