5.8S ribosomal RNA

5.8S ribosomal RNA
Predicted secondary structure and sequence conservation of 5.8S ribosomal RNA
Identifiers
Symbol5_8S_rRNA
RfamRF00002
Other data
RNA typeGene; rRNA
Domain(s)Eukaryota; Archaea[citation needed]
GOGO:0003735 GO:0005840
SOSO:0000375
PDB structuresPDBe

In molecular biology, the 5.8S ribosomal RNA (5.8S rRNA) is a non-coding RNA component of the large subunit of the eukaryotic ribosome and so plays an important role in protein translation. It is transcribed by RNA polymerase I as part of the 45S precursor that also contains 18S and 28S rRNA. Its function is thought to be in ribosome translocation.[1] It is also known to form covalent linkage to the p53 tumour suppressor protein.[2] 5.8S rRNA can be used as a reference gene for miRNA detection.[3] The 5.8S ribosomal RNA is used to better understand other rRNA processes and pathways in the cell.[4]

The 5.8S rRNA is homologous to the 5' end of non-eukaryotic LSU rRNA. In eukaryotes, the insertion of ITS2 breaks LSU rRNA into 5.8S and 28S rRNAs.[5] Some flies have their 5.8 rRNA further split into two pieces.[6]

  1. ^ Abou Elela S, Nazar RN (May 1997). "Role of the 5.8S rRNA in ribosome translocation". Nucleic Acids Research. 25 (9): 1788–1794. doi:10.1093/nar/25.9.1788. PMC 146658. PMID 9108162.
  2. ^ Fontoura BM, Atienza CA, Sorokina EA, Morimoto T, Carroll RB (June 1997). "Cytoplasmic p53 polypeptide is associated with ribosomes". Molecular and Cellular Biology. 17 (6): 3146–3154. doi:10.1128/MCB.17.6.3146. PMC 232167. PMID 9154813.
  3. ^ Shi R, Chiang VL (October 2005). "Facile means for quantifying microRNA expression by real-time PCR". BioTechniques. 39 (4): 519–525. doi:10.2144/000112010. PMID 16235564.
  4. ^ Nazar RN (June 1984). "The ribosomal 5.8S RNA: eukaryotic adaptation or processing variant?". Canadian Journal of Biochemistry and Cell Biology. 62 (6): 311–320. doi:10.1139/o84-044. PMID 6380683.
  5. ^ Lafontaine, D. L. J.; Tollervey, D. (2001). "The function and synthesis of ribosomes". Nature Reviews Molecular Cell Biology. 2 (7): 514–520. doi:10.1038/35080045. hdl:1842/729. PMID 11433365. S2CID 2637106.
  6. ^ Shimada, T. (August 1992). "Distribution of split 5.8S ribosomal RNA in Diptera". Insect Molecular Biology. 1 (1): 45–48. doi:10.1111/j.1365-2583.1993.tb00076.x. PMID 1343775. S2CID 46570307.