| Grade II race | |
| Location | Churchill Downs Louisville, Kentucky, United States |
|---|---|
| Inaugurated | 2004 |
| Race type | Thoroughbred - Flat racing |
| Sponsor | Twinspires (2024) |
| Website | Churchill Downs |
| Race information | |
| Distance | 1+1⁄16 miles (1.7 km) |
| Surface | Dirt |
| Track | left-handed |
| Qualification | Four-year-olds and up |
| Weight | 123 lb (56 kg) with allowances |
| Purse | US$750,000 (since 2024) |
The Alysheba Stakes is a Grade II American Thoroughbred horse race for horses aged three and older over a distance of 1+1⁄16 miles (1.7 km). It is held annually in early May on the dirt on the Kentucky Oaks day meeting at Churchill Downs in Louisville, Kentucky, during the spring meeting.