| Anion-transporting ATPase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | ArsA_ATPase | ||||||||
| Pfam | PF02374 | ||||||||
| Pfam clan | CL0023 | ||||||||
| SCOP2 | 1f48 / SCOPe / SUPFAM | ||||||||
| TCDB | 3.A.4 | ||||||||
| |||||||||
| ArsB | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | ArsB | ||||||||
| Pfam | PF02040 | ||||||||
| Pfam clan | CL0182 | ||||||||
| InterPro | IPR000802 | ||||||||
| TCDB | 3.A.4 | ||||||||
| |||||||||
| ArsC | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 yffb (pa3664) protein | |||||||||
| Identifiers | |||||||||
| Symbol | ArsC | ||||||||
| Pfam | PF03960 | ||||||||
| Pfam clan | CL0172 | ||||||||
| InterPro | IPR006660 | ||||||||
| SCOP2 | 1i9d / SCOPe / SUPFAM | ||||||||
| |||||||||
| ArsD | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | ArsD | ||||||||
| Pfam | PF06953 | ||||||||
| Pfam clan | CL0172 | ||||||||
| InterPro | IPR010712 | ||||||||
| |||||||||
| ArsR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | ArsR | ||||||||
| Pfam | PF09824 | ||||||||
| Pfam clan | CL0123 | ||||||||
| InterPro | IPR018334 | ||||||||
| SCOP2 | a.4.5.5 / SCOPe / SUPFAM | ||||||||
| |||||||||
In molecular biology, the ars operon is an operon found in several bacterial taxon. It is required for the detoxification of arsenate, arsenite, and antimonite.[1] This system transports arsenite and antimonite out of the cell. The pump is composed of two polypeptides, the products of the arsA and arsB genes. This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite before it is extruded. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance. ArsC is an approximately 150-residue arsenate reductase that uses reduced glutathione (GSH) to convert arsenate to arsenite with a redox active cysteine residue in the active site. ArsC forms an active quaternary complex with GSH, arsenate, and glutaredoxin 1 (Grx1). The three ligands must be present simultaneously for reduction to occur.[2]
- ^ Carlin A, Shi W, Dey S, Rosen BP (February 1995). "The ars operon of Escherichia coli confers arsenical and antimonial resistance". J. Bacteriol. 177 (4): 981–6. doi:10.1128/jb.177.4.981-986.1995. PMC 176692. PMID 7860609.
- ^ Liu J, Rosen BP (August 1997). "Ligand interactions of the ArsC arsenate reductase". J. Biol. Chem. 272 (34): 21084–9. doi:10.1074/jbc.272.34.21084. PMID 9261111.