Bacillus virus Φ29 | |
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An illustration of Φ29's head based on electron microscopy data EMDB-2162 | |
Virus classification | |
(unranked): | Virus |
Realm: | Duplodnaviria |
Kingdom: | Heunggongvirae |
Phylum: | Uroviricota |
Class: | Caudoviricetes |
Order: | Caudovirales |
Family: | Salasmaviridae |
Genus: | Salasvirus |
Species: | Bacillus virus Φ29
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Bacillus virus Φ29 (bacteriophage Φ29) is a double-stranded DNA (dsDNA) bacteriophage with a prolate icosahedral head and a short tail that belongs to the genus Salasvirus, order Caudovirales, and family Salasmaviridae.[2][3] They are in the same order as phages PZA, Φ15, BS32, B103, M2Y (M2), Nf, and GA-1.[4][5] First discovered in 1965, the Φ29 phage is the smallest Bacillus phage isolated to date and is among the smallest known dsDNA phages.[2][3]
Φ29 has a unique DNA packaging motor structure that employs prohead packaging RNA (pRNA) to guide the translocation of the phage genome during replication. This novel structure system has inspired ongoing research in nanotechnology, drug delivery, and therapeutics.[6][7][8][9]
In nature, the Φ29 phage infects Bacillus subtilis, a species of gram-positive, endospore-forming bacteria that is found in soil, as well as the gastrointestinal tracts of various marine and terrestrial organisms, including human beings.[10]
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