Bone-marrow-derived macrophage (BMDM) refers to macrophage cells that are generated in a research laboratory from mammalian bone marrow cells.[1][2][3] BMDMs can differentiate into mature macrophages in the presence of growth factors and other signaling molecules.[1][2] Undifferentiated bone marrow cells are cultured in the presence of macrophage colony-stimulating factor (M-CSF; CSF-1).[3] M-CSF is a cytokine and growth factor that is responsible for the proliferation and commitment of myeloidprogenitors into monocytes (which then mature into macrophages).[3][4] Macrophages have a wide variety of functions in the body including phagocytosis of foreign invaders and other cellular debris, releasing cytokines to trigger immune responses, and antigen presentation.[2] BMDMs provide a large homogenous population of macrophages that play an increasingly important role in making macrophage-related research possible and financially feasible.[5]