C7.GAT protein

This article includes a list of references, related reading, or external links, but its sources remain unclear because it lacks inline citations. Please help improve this article by introducing more precise citations. (December 2009) (Learn how and when to remove this message)

This article relies largely or entirely on a single source. Relevant discussion may be found on the talk page. Please help improve this article by introducing citations to additional sources. Find sources: "C7.GAT protein" – news · newspapers · books · scholar · JSTOR (June 2024)

The C7.GAT protein is a zinc finger protein based on the C7 protein (itself based on the murine Zif268). It features an alternative zinc finger 3 alpha helix sequence, preventing the target site overlap caused by the aspartic acid residue of the finger 3 of C7. The sequence of this third finger is TSG-N-LVR according to the single letter amino acid code. As the name suggest, the target site of finger 3 is altered to 5'-GAT-3', giving the overall protein a target of 5'-GCGTGGGAT-3'.

C7.GAT is used in studies investigating the effects of altering zinc finger alpha helix sequence on the target of the altered zinc finger, as well as the affinity and specificity of these proteins to their targets.