Calcofluor-white

Calcofluor-white or CFW is a fluorescent blue dye used in biology and textiles. It binds to 1–3 beta and 1–4 beta polysaccharides of chitin and cellulose that are present in cell walls on fungi, plants, and algae.

The fluorescent staining of yeast with calcofluor-white. The cell walls fluoresce to a vivid blue color.

In plant cell biology research, it is used for the staining of cell walls of both algae and higher plants.[1][2] It is also useful in medicine and animal biology as a sensitive tool for the visualization and identification of fungi in the tissue.[1][3][4]

  1. ^ a b Herth, W.; E. Schnepf (1980). "The fluorochrome, calcofluor white, binds oriented to structural polysaccharide fibrils". Protoplasma. 105 (1–2). Springer: 129–133. doi:10.1007/BF01279855. ISSN 0033-183X. S2CID 37459059.
  2. ^ Bidhendi, AJ; Chebli, Y; Geitmann, A (May 2020). "Fluorescence Visualization of Cellulose and Pectin in the Primary Plant Cell Wall". Journal of Microscopy. 278 (3): 164–181. doi:10.1111/jmi.12895. PMID 32270489. S2CID 215619998.
  3. ^ Hoch, H.C.; C.D. Galvani; D.H. Szarowski; J.N. Turner (2005). "Two new fluorescent dyes applicable for visualization of fungal cell walls". Mycologia. 97 (3): 580–588. doi:10.3852/mycologia.97.3.580. ISSN 0027-5514. PMID 16392246.
  4. ^ Burns, Edward R.; Wittner M.; Faskowitz F. (5 April 2005). "Method for detecting chitin-containing organisms - Patent 6875421". Retrieved 10 November 2010.