Fixative solution
Carnoy's solution is a fixative composed of 60% ethanol , 30% chloroform and 10% glacial acetic acid , 1 gram of ferric chloride.[ 1] [ 2]
Carnoy's solution is also the name of a different fixation composed of ethanol and glacial acetic acid (3:1).[ 3] [ 4] [ 5]
The invention of Carnoy's solution is attributed to Jean-Baptiste Carnoy, a pioneering 19th century cytologist.[ 6]
^ "MSDS :: Carnoy's Solution (Fixative)" . Archived from the original on 2009-03-27. Retrieved 13 Jan 2009 .
^ Carnoy J. B. (1887). "Appendice Les Globule Polaires de L'Ascaris Clavata" . La Cellule RECUEIL DE CYTOLOGIE ET d'HISTOLOGIE GÉNÉRALE . 3 : 276.
^ Tjio JH, Whang J (1962). "Chromosome Preparatons of Bone Marrow Cells without PriorIn VitroCulture orIn VivoColchicine Administration". Stain Technology . 37 : 17–20. doi :10.3109/10520296209114563 . PMID 13921436 .
^ Mazia D, Brewer PA, Alfert MA (1953). "The Cytochemical Staining and Measurement of Protein with Mercuric Bromphenol Blue" . The Biological Bulletin . 104 (1): 57–67. doi :10.2307/1538691 . JSTOR 1538691 .
^ Utevsky S, Kovalenko N, Doroshenko K, Petrauskienė L, Klymenko V (2009). "Chromosome numbers for three species of medicinal leeches (Hirudo spp.)". Systematic Parasitology . 74 (2): 95–102. doi :10.1007/s11230-009-9198-2 . PMID 19731093 . S2CID 7947757 .
^ Ireland, Robert; Yeung, Chuen Albert YeungChuen Albert (2020-04-23), Yeung, Chuen Albert (ed.), "Carnoy's solution" , A Dictionary of Dentistry , Oxford University Press, doi :10.1093/acref/9780191828621.001.0001 , ISBN 978-0-19-182862-1 , retrieved 2023-04-10