Charles Gardner may refer to:
- Charles Gardner (botanist) (1896–1970), Australian botanist
- Charles Gardner (Australian cricketer) (1908–2001), Australian cricketer
- Charles Gardner (South African cricketer) (1913–2001), South African cricketer
- Charles Gardner (politician) (1828–1917), politician from Minnesota Territory
- Charles Gardner (Medal of Honor) (1844–1895), German-born Indian Wars Medal of Honor recipient
- Charles J. Gardner (1843–1901), American politician from New York
- Charles N. Gardner (1845–1919), American Civil War soldier and Medal of Honor recipient
- Alfred Charles Gardner (1880–1952), Scottish engineer
- Charles Bruce-Gardner (1887–1960), English industrialist
- Charles Gardner Radbourn (1854–1897), American professional baseball pitcher
- Bertie Charles Gardner (1884–1972), British-Canadian banker
- Charles Gairdner (1898–1983), British Army general
- Charles Gardner Geyh, law professor at Indiana University
- Dick Gardner (1913–1997), real name Charles, English footballer