Culturomics is the high-throughputcell culture of bacteria that aims to comprehensively identify strains or species in samples obtained from tissues such as the human gut or from the environment.[1][2][3] This approach was conceived as an alternative, complementary method to metagenomics, which relies on the presence of homologoussequences to identify new bacteria.[3] Due to the limited phylogenetic information available on bacteria, metagenomic data generally contains large amounts of "microbial dark matter", sequences of unknown origin.[4] Culturomics provides some of the missing gaps with the added advantage of enabling the functional study of the generated cultures. Its main drawback is that many bacterial species remain effectively uncultivable until their growth conditions are better understood. Therefore, optimization of the culturomics approach has been done by improving culture conditions.[5][6]