DNA polymerase II

This article is about the DNA polymerase. For the RNA polymerase, see RNA polymerase II.
DNA Polymerase II
Crystal structure of DNA pol II (based on PDB entry 3K5M)
Identifiers
OrganismEscherichia coli
(str. K-12 substr. MG1655)
SymbolpolB
Entrez944779
PDB3K5M
RefSeq (Prot)NP_414602.1
UniProtP21189
Other data
EC number2.7.7.7
Chromosomegenome: 0.06 - 0.07 Mb
Search for
StructuresSwiss-model
DomainsInterPro

DNA polymerase II (also known as DNA Pol II or Pol II) is a prokaryotic DNA-dependent DNA polymerase encoded by the PolB gene.[1]

DNA Polymerase II is an 89.9-kDa protein and is a member of the B family of DNA polymerases. It was originally isolated by Thomas Kornberg in 1970, and characterized over the next few years.[2][3][4] The in vivo functionality of Pol II is under debate, yet consensus shows that Pol II is primarily involved as a backup enzyme in prokaryotic DNA replication. The enzyme has 5′→3′ DNA synthesis capability as well as 3′→5′ exonuclease proofreading activity. DNA Pol II interacts with multiple binding partners common with DNA Pol III in order to enhance its fidelity and processivity.[1]

  1. ^ a b Banach-Orlowska M, Fijalkowska IJ, Schaaper RM, Jonczyk P (October 2005). "DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli". Molecular Microbiology. 58 (1): 61–70. doi:10.1111/j.1365-2958.2005.04805.x. PMID 16164549.
  2. ^ Kornberg T, Gefter ML (September 1970). "DNA synthesis in cell-free extracts of a DNA polymerase-defective mutant". Biochemical and Biophysical Research Communications. 40 (6): 1348–55. doi:10.1016/0006-291X(70)90014-8. PMID 4933688.
  3. ^ Moses RE, Richardson CC (December 1970). "A new DNA polymerase activity of Escherichia coli. I. Purification and properties of the activity present in E. coli polA1". Biochemical and Biophysical Research Communications. 41 (6): 1557–64. doi:10.1016/0006-291X(70)90565-6. PMID 4922636.
  4. ^ Knippers R (December 1970). "DNA polymerase II". Nature. 228 (5276): 1050–3. Bibcode:1970Natur.228.1050K. doi:10.1038/2281050a0. PMID 4921664. S2CID 4211529.