Dorothy Stafford | |
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| Born | 1 October 1526 England |
| Died | 22 September 1604 (aged 77) |
| Buried | St. Margaret's Church, Westminster, London |
| Noble family | Stafford |
| Spouse(s) | Sir William Stafford |
| Issue | Sir Edward Stafford William Stafford Sir John Stafford Elizabeth Stafford Ursula Stafford Dorothy Stafford |
| Father | Henry Stafford, 1st Baron Stafford |
| Mother | Ursula Pole |
| Occupation | Mistress of the Robes to Elizabeth I |
Dorothy Stafford, Lady Stafford (1 October 1526 – 22 September 1604) was an English noblewoman, and an influential person at the court of Queen Elizabeth I of England, to whom she served as Mistress of the Robes. Dorothy Stafford was the second wife of Sir William Stafford, widower of Mary Boleyn. She and her family sought exile in Geneva during the reign of Mary I to escape the persecution of their Protestant religion. The Protestant reformer John Calvin stood as godfather to her youngest son.