E is the third note and the fifth semitone of the C major scale, and mi in fixed-do solfège. It has enharmonic equivalents of F♭ [(F-flat) which is by definition a diatonic semitone above E♭] and D (D-double sharp), amongst others.
When calculated in equal temperament with a reference of A above middle C as 440 Hz, the frequency of Middle E (E4) is approximately 329.628 Hz.[1] See pitch (music) for a discussion of historical variations in frequency.