Electron-transfer dissociation

An ion trap mass spectrometer with electron transfer dissociation capability
Peptide fragmentation notation

Electron-transfer dissociation (ETD) is a method of fragmenting multiply-charged gaseous macromolecules in a mass spectrometer between the stages of tandem mass spectrometry (MS/MS).[1] Similar to electron-capture dissociation, ETD induces fragmentation of large, multiply-charged cations by transferring electrons to them.[2] ETD is used extensively with polymers and biological molecules such as proteins and peptides for sequence analysis.[3] Transferring an electron causes peptide backbone cleavage into c- and z-ions while leaving labile post translational modifications (PTM) intact.[4] The technique only works well for higher charge state peptide or polymer ions (z>2).[2] However, relative to collision-induced dissociation (CID), ETD is advantageous for the fragmentation of longer peptides or even entire proteins.[5] This makes the technique important for top-down proteomics. The method was developed by Hunt and coworkers at the University of Virginia.[6]

  1. ^ Dass, Chhabil (2007). Fundamentals of Contemporary Mass Spectrometry. Hoboken, New Jersey: John Wiley & Sons. p. 128. ISBN 978-0-470-11848-1.
  2. ^ a b Hart-Smith, Gene (2014-01-15). "A review of electron-capture and electron-transfer dissociation tandem mass spectrometry in polymer chemistry". Analytica Chimica Acta. Polymer Mass Spectrometry. 808: 44–55. Bibcode:2014AcAC..808...44H. doi:10.1016/j.aca.2013.09.033. PMID 24370092.
  3. ^ Brodbelt, Jennifer S. (2015-12-11). "Ion Activation Methods for Peptides and Proteins". Analytical Chemistry. 88 (1): 30–51. doi:10.1021/acs.analchem.5b04563. PMC 5553572. PMID 26630359.
  4. ^ Coon, Joshua J.; Syka, John E.P.; Shabanowitz, Jeffrey; Hunt, Donald F. (April 2005). "Tandem Mass Spectrometry for Peptide and Protein Sequence Analysis". BioTechniques. 38 (4): 519, 521, 523. doi:10.2144/05384te01. PMID 15884666.
  5. ^ Good, David M.; Wirtala, Matthew; McAlister, Graeme C.; Coon, Joshua J. (2007-11-01). "Performance Characteristics of Electron Transfer Dissociation Mass Spectrometry". Molecular & Cellular Proteomics. 6 (11): 1942–1951. doi:10.1074/mcp.M700073-MCP200. ISSN 1535-9476. PMID 17673454.
  6. ^ US patent 7534622, Donald F. Hunt, Joshua J. Coon, John E.P. Syka, Jarrod A. Marto, "Electron transfer dissociation for biopolymer sequence mass spectrometric analysis", issued 2009-05-19