State highway in Florida, U.S.
This article is about the Florida state highway. For the U.S. highway with the same number, see
U.S. Route 331.
State Road 331 (SR 331) is a short state highway running south and east of Gainesville, although it once stretched as far south as Williston. It also serves as a truck route for State Roads 24, 26, and 121 and US 441. Despite skirting the Gainesville City Limits, SR 331 runs north and south as a four-lane divided rural highway.
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