Fluorescence microscope

An upright fluorescence microscope (Olympus BX61) with the fluorescence filter cube turret above the objective lenses, coupled with a digital camera
Fluorescence and confocal microscopes operating principle

A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.[1][2] "Fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image.[3]

  1. ^ Spring KR, Davidson MW. "Introduction to Fluorescence Microscopy". Nikon MicroscopyU. Retrieved 24 October 2024.
  2. ^ "The Fluorescence Microscope". Microscopes—Help Scientists Explore Hidden Worlds. The Nobel Foundation. Retrieved 28 September 2008.
  3. ^ Juan Carlos Stockert, Alfonso Blázquez-Castro (2017). Fluorescence Microscopy in Life Sciences. Bentham Science Publishers. ISBN 978-1-68108-519-7. Archived from the original on 14 May 2019. Retrieved 17 December 2017.