Genomic library

A genomic library is a collection of overlapping DNA fragments that together make up the total genomic DNA of a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase.[1] Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.[2]

There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find the desired number of clones necessary for full genome coverage.[3]

Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.[4][5]

  1. ^ Losick, Richard; Watson, James D.; Tania A. Baker; Bell, Stephen; Gann, Alexander; Levine, Michael W. (2008). Molecular biology of the gene. San Francisco: Pearson/Benjamin Cummings. ISBN 978-0-8053-9592-1.
  2. ^ Russell, David W.; Sambrook, Joseph (2001). Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory. ISBN 978-0-87969-577-4.
  3. ^ Hartwell, Leland (2008). Genetics: from genes to genomes. Boston: McGraw-Hill Higher Education. ISBN 978-0-07-284846-5.
  4. ^ Muse, Spencer V.; Gibson, Greg (2004). A primer of genome science. Sunderland, Mass: Sinauer Associates. ISBN 978-0-87893-232-0.
  5. ^ Henry RJ, Edwards M, Waters DL, et al. (November 2012). "Application of large-scale sequencing to marker discovery in plants". J. Biosci. 37 (5): 829–41. doi:10.1007/s12038-012-9253-z. PMID 23107919. S2CID 14457634.