Gnotobiosis (from Greek roots gnostos "known" and bios "life") refers to an engineered state of an organism in which all forms of life (i.e., microorganisms) in or on it, including its microbiota, have been identified.[1] The term gnotobiotic organism, or gnotobiote, can refer to a model organism that is colonized with a specific community of known microorganisms (isobiotic or defined flora animal) or that contains no microorganisms (germ-free) often for experimental purposes.[2][3][4][5] The study of gnotobiosis and the generation of various types of gnotobiotic model organisms as tools for studying interactions between host organisms and microorganisms is referred to as gnotobiology.[2]
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