GsMTx-4

M-theraphotoxin-Gr1a
Identifiers
OrganismGrammostola rosea
SymbolGsMTx-4
PDB1LU8
UniProtQ7YT39
Search for
StructuresSwiss-model
DomainsInterPro
GsMTx-4
Names
IUPAC name
glycyl-cysteinyl-leucyl-alpha-glutamyl-phenylalanyl-tryptophyl-tryptophyl-lysyl-cysteinyl-asparagyl-prolyl-asparagyl-alpha-aspartyl-alpha-aspartyl-lysyl-cysteinyl-cysteinyl-arginyl-prolyl-lysyl-leucyl-lysyl-cysteinyl-seryl-lysyl-leucyl-phenylalanyl-lysyl-leucyl-cysteinyl-asparagyl-phenylalanyl-seryl-phenylalaninamide (2->17),(9->23),(16->30)-tris(disulfide)
Other names
  • M-TRTx-Gr1a
  • M-theraphotoxin-Gr1a
Identifiers
3D model (JSmol)
  • InChI=1S/C185H273N49O45S6/c1-98(2)72-121-160(255)203-114(54-27-33-65-188)156(251)229-141-96-284-283-95-140-178(273)225-134(84-147(195)239)184(279)234-71-39-60-144(234)182(277)224-131(83-146(194)238)170(265)222-133(86-151(245)246)172(267)223-132(85-150(243)244)171(266)206-116(56-29-35-67-190)158(253)230-142(97-285-282-94-139(177(272)221-130(82-145(193)237)169(264)218-127(79-105-46-19-12-20-47-105)166(261)226-136(91-236)174(269)211-120(152(196)247)76-102-40-13-9-14-41-102)231-163(258)124(75-101(7)8)214-153(248)112(52-25-31-63-186)204-164(259)125(77-103-42-15-10-16-43-103)217-162(257)123(74-100(5)6)213-154(249)113(53-26-32-64-187)207-173(268)135(90-235)227-179(141)274)180(275)232-138(176(271)210-119(58-37-69-199-185(197)198)183(278)233-70-38-59-143(233)181(276)209-117(155(250)212-121)57-30-36-68-191)93-281-280-92-137(202-148(240)87-192)175(270)215-122(73-99(3)4)161(256)208-118(61-62-149(241)242)159(254)216-126(78-104-44-17-11-18-45-104)165(260)219-129(81-107-89-201-111-51-24-22-49-109(107)111)168(263)220-128(80-106-88-200-110-50-23-21-48-108(106)110)167(262)205-115(157(252)228-140)55-28-34-66-189/h9-24,40-51,88-89,98-101,112-144,200-201,235-236H,25-39,52-87,90-97,186-192H2,1-8H3,(H2,193,237)(H2,194,238)(H2,195,239)(H2,196,247)(H,202,240)(H,203,255)(H,204,259)(H,205,262)(H,206,266)(H,207,268)(H,208,256)(H,209,276)(H,210,271)(H,211,269)(H,212,250)(H,213,249)(H,214,248)(H,215,270)(H,216,254)(H,217,257)(H,218,264)(H,219,260)(H,220,263)(H,221,272)(H,222,265)(H,223,267)(H,224,277)(H,225,273)(H,226,261)(H,227,274)(H,228,252)(H,229,251)(H,230,253)(H,231,258)(H,232,275)(H,241,242)(H,243,244)(H,245,246)(H4,197,198,199)
    Key: WVDNTWXIIKNMHY-UHFFFAOYSA-N
  • [H]NCC(=O)N[C@H]1CSSC[C@@H]2NC(=O)[C@@H]3CSSC[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC4=CC=CC=C4)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CSSC[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC4=CNC5=C4C=CC=C5)NC(=O)[C@H](CC4=CNC5=C4C=CC=C5)NC(=O)[C@H](CC4=CC=CC=C4)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC1=O)C(=O)N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N3)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(N)=O
Properties
C185H273N49O45S6
Molar mass 4095.88 g·mol−1
1 mg/mL
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

Grammostola mechanotoxin #4 (GsMTx-4, GsMTx4, GsMTx-IV), also known as M-theraphotoxin-Gr1a (M-TRTX-Gr1a), is a neurotoxin isolated from the venom of the spider Chilean rose tarantula Grammostola spatulate (or Grammostola rosea).[1] This amphiphilic peptide, which consists of 35 amino acids, belongs to the inhibitory cysteine knot (ICK) peptide family.[2] It reduces mechanical sensation by inhibiting mechanosensitive channels (MSCs).[3]

GsMTx-4 also serves as a cationic antimicrobial peptide against Gram-positive bacteria.[4]

  1. ^ Suchyna TM, Johnson JH, Hamer K, Leykam JF, Gage DA, Clemo HF, et al. (May 2000). "Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels". The Journal of General Physiology. 115 (5): 583–598. doi:10.1085/jgp.115.5.583. PMC 2217226. PMID 10779316.
  2. ^ Oswald RE, Suchyna TM, McFeeters R, Gottlieb P, Sachs F (September 2002). "Solution structure of peptide toxins that block mechanosensitive ion channels". The Journal of Biological Chemistry. 277 (37): 34443–34450. doi:10.1074/jbc.M202715200. PMID 12082099.
  3. ^ Park SP, Kim BM, Koo JY, Cho H, Lee CH, Kim M, et al. (July 2008). "A tarantula spider toxin, GsMTx4, reduces mechanical and neuropathic pain". Pain. 137 (1): 208–217. doi:10.1016/j.pain.2008.02.013. PMID 18359568. S2CID 23399357.
  4. ^ Suchyna TM (November 2017). "Piezo channels and GsMTx4: Two milestones in our understanding of excitatory mechanosensitive channels and their role in pathology". Progress in Biophysics and Molecular Biology. Cardiac Mechanics and Electrics: it takes two to tango. 130 (Pt B): 244–253. doi:10.1016/j.pbiomolbio.2017.07.011. PMC 5716857. PMID 28778608.