Guido is a male given name. It may also refer to:
Guido may also refer to:
- Guido (slang), slang term for Italian-American
- Guido (surname)
- GUIDO music notation, a computer music notation format
- Guido Island, Wilhelm Archipelago, Antarctica
- 120361 Guido, an asteroid
- GUIDO, a Guidance Officer or flight controller in Apollo space missions
- Guido, a character in Disney and Pixar's Cars franchise.