Holby City series 23

The twenty-third and final series of the British medical drama television series Holby City began airing on BBC One in the United Kingdom on 6 April 2021. The series consists of 50 episodes, which focus on the professional and personal lives of medical and ancillary staff at the fictional Holby City Hospital. Seán Gleeson serves as the series producer. A new theme tune was introduced in the series, but was negatively received from viewers. As the series was filmed during the COVID-19 pandemic, it had to comply with safety protocols, including social distancing and reduced people on-set. Early in the series, actors Dawn Steele and Trieve Blackwood-Cambridge isolated and became a work bubble for two episodes to film close proximity scenes. The series was promoted through multiple trailers and behind-the-scenes videos.

The show's cancellation was announced in June 2021 as part of plans from the BBC to make more nationwide programming. Holby City concludes with this series and the finale first airs on 29 March 2022. There was a wide backlash to the news from critics, fans and past and present cast members. A petition was launched to save the show, reaching over 43,700 signatures. Filming for the show concluded in December 2021 and pays homage to the National Health Service (NHS) and the show's cast and crew.

Fifteen actors continued their roles from the previous series with three regular characters departing during this series. Multiple former cast members reprised their roles, including some for the show's final episodes. Rosie Marcel (Jac Naylor) and Belinda Owusu (Nicky McKendrick) returned following breaks, while 14 actors returned for guest stints of varying lengths. Four new regular characters were introduced in series 23. Additionally, several recurring characters and multiple guest stars feature in the series.