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Stadia during an ITP separation of a mix of two analytes.
White: leading electrolyte; gray: terminating electrolyte; hatched: the analytes

The self-sharpening effect in ITP: due to a difference in electrical field, an ion will move faster when it comes in the previous zone, and slower when it comes in the next zone. Therefore, it will return to its own zone. Below the corresponding electrical field for each zone

Isotachophoresis (ITP) is a technique in analytical chemistry used for selective separation and concentration of ionic analytes. It is a form of electrophoresis; charged analytes are separated based on ionic mobility, a quantity which tells how fast an ion migrates through an electric field.