James or Jim Holt may refer to:
- James Holt (MP) (1647–1713), English MP for Lancashire
- James Maden Holt (1829–1911), English Conservative Party politician, MP for North East Lancashire 1868–1880
- James Holt (American football) (born 1986), American gridiron player
- Jim Holt (philosopher), American philosopher, author and essayist
- James Holt (scholar), British Latter-day Saint scholar
- James Holt (historian) (1922–2014), British professor of mediaeval history
- James Richard Holt (1931–1991), British politician
- Jim Holt (actor) (born 1959), British/Australian actor
- Jim Holt (baseball) (1944–2019), American baseball player
- Jim Holt (Arkansas politician) (born 1965), Arkansas politician