John Wolfe may refer to:
- John Wolfe (printer) (1548?–1601), English bookseller and printer
- John Wolfe, 2nd Viscount Kilwarden (1769–1830), Irish peer
- Jack A. Wolfe (1936–2005), American paleontologist
- John Bascom Wolfe (1904–1988), American social and behavioral psychologist
- John Clay Wolfe (born 1972), American radio personality and entrepreneur
- John Wolfe Jr. (born 1954), Tennessee politician
- John P. Wolfe (born 1970), American chemist
- John Richard Wolfe (1832-1915), Irish missionary who served in China
- John Thomas Wolfe (1955–1995), veterinarian and Canadian provincial politician
- John T. Wolfe Jr. (born 1942), president of Savannah State College