Jota may refer to:
- Iota (Ι, ι), the name of the 9th letter in the Greek alphabet;
- (figuratively) Something very small, based on the fact that the letter Iota (lat. i) is the smallest character in the alphabet;
- The name of the letter J in Spanish and Portuguese;
- Jota (music), a type of Spanish music and dance;
- Jota (food), an Istrian bean-sauerkraut stew;
- Laverda Jota, an Italian motorcycle;
- Jota Sport, an English car racing team;
- Jamboree on the Air (JOTA), an international amateur radio event organised by Scouts;
- Lamborghini Diablo SE30 Jota, a circuit racing version of the Lamborghini Diablo 30th anniversary edition;
- Lamborghini Miura P400 Jota, a model of the Lamborghini Miura;
- Mazda Jota MX-5 GT, a model of the Mazda MX-5;
- Jota Aviation, a British airline.