Langendorff heart

"Langendorff procedure" redirects here. For other prepared in vitro organs, see Retrograde perfusion.
Native records of contractile activity of the left ventricle of isolated rat heart perfused under Langendorff technique. Curve A - contractile function of the heart is greatly depressed after ischemia-reperfusion. Curve B - a set of short ischemic episodes (ischemic preconditioning) before prolonged ischemia provides functional recovery of contractile activity of the heart at reperfusion.

The Langendorff heart or isolated perfused heart assay is an ex vivo technique used in pharmacological and physiological research using animals and also humans.[1] Named after the German physiologist Oskar Langendorff, this technique allows the examination of cardiac contractile strength and heart rate without the complications of an intact animal or human.[2] After more than 100 years, this method is still being used.[3]

  1. ^ Tseng CE, Miranda E, Di Donato F, Boutjdir M, Rashbaum W, Chan EK, et al. (February 1999). "mRNA and protein expression of SSA/Ro and SSB/La in human fetal cardiac myocytes cultured using a novel application of the Langendorff procedure". Pediatric Research. 45 (2): 260–269. doi:10.1203/00006450-199902000-00018. PMID 10022600.
  2. ^ Bell RM, Mocanu MM, Yellon DM (June 2011). "Retrograde heart perfusion: the Langendorff technique of isolated heart perfusion". Journal of Molecular and Cellular Cardiology. 50 (6): 940–50. doi:10.1016/j.yjmcc.2011.02.018. PMID 21385587.
  3. ^ King DR, Hardin KM, Hoeker GS, Poelzing S (September 2022). "Reevaluating methods reporting practices to improve reproducibility: an analysis of methodological rigor for the Langendorff whole heart technique". American Journal of Physiology. Heart and Circulatory Physiology. 323 (3): H363–H377. doi:10.1152/ajpheart.00164.2022. PMC 9359653. PMID 35749719.