Loop-mediated isothermal amplification

Loop-mediated isothermal amplification (LAMP) process[1]

Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA[2] for diagnostic purposes and a low-cost alternative to detect certain diseases.[3] LAMP is an isothermal nucleic acid amplification technique. In contrast to the polymerase chain reaction (PCR) technology, in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature, and does not require a thermal cycler. LAMP was invented in 1998 by Eiken Chemical Company in Tokyo.[1] Reverse transcription loop-mediated isothermal amplification (RT-LAMP) combines LAMP with a reverse transcription step to allow the detection of RNA.

  1. ^ a b M. Soroka, B. Wasowicz, A. Rymaszewska: Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR? In: Cells. Volume 10, issue 8, July 2021, p. , doi:10.3390/cells10081931, PMID 34440699, PMC 8393631.
  2. ^ US patent 6410278, Notomi T, Hase T, "Process for synthesizing nucleic acid", published 2002-06-25, assigned to Eiken Kagaku Kabushiki Kaisha 
  3. ^ Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000). "Loop-mediated isothermal amplification of DNA". Nucleic Acids Res. 28 (12): 63e–63. doi:10.1093/nar/28.12.e63. PMC 102748. PMID 10871386.