Louisa Holthuysen | |
|---|---|
 Miniature portrait of Louisa Holthuysen with her Maltese lion dog, c. 1840-1860 | |
| Born | 28 November 1824 |
| Died | 30 January 1895 |
| Nationality | Kingdom of the Netherlands |
Sandrina Louisa Geertruida Holthuysen (1824 – 1895) was a Dutch art collector and founder of the Museum Willet-Holthuysen in Amsterdam.