Overlap extension polymerase chain reaction

The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap extension / Splicing by overhang extension (SOE) PCR. It is used to assemble multiple smaller double stranded DNA fragments into a larger DNA sequence. OE-PCR is widely used to insert mutations at specific points in a sequence or to assemble custom DNA sequence from smaller DNA fragments into a larger polynucleotide.^[1]

^ Higuchi R, Krummel B, Saiki R (1988). "A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions". Nucleic Acids Res. 16 (15): 7351–67. doi:10.1093/nar/16.15.7351. PMC 338413. PMID 3045756.

[1] Higuchi R, Krummel B, Saiki R (1988). "A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions". Nucleic Acids Res. 16 (15): 7351–67. doi:10.1093/nar/16.15.7351. PMC 338413. PMID 3045756.

[1]