Perls Prussian blue

Cerebrospinal fluid specimen stained with Perls Prussian blue showing iron containing macrophage (stained blue) surrounded by erythrocytes (stained red)

In histology, histopathology, and clinical pathology, Perls Prussian blue is a commonly used method to detect the presence of iron in tissue or cell samples.[1]: 235 [2][3][4] Perls Prussian Blue derives its name from the German pathologist Max Perls (1843–1881), who described the technique in 1867.[2] The method does not involve the application of a dye, but rather causes the pigment Prussian blue to form directly within the tissue.[5] The method stains mostly iron in the ferric state which includes ferritin and hemosiderin, rather than iron in the ferrous state.[6]

  1. ^ Cite error: The named reference Bancroft and Stevens, 2008 was invoked but never defined (see the help page).
  2. ^ a b Cite error: The named reference Meguro et al., 2007 was invoked but never defined (see the help page).
  3. ^ Cite error: The named reference Parmley et al., 1978 was invoked but never defined (see the help page).
  4. ^ Cite error: The named reference Theil, 2012 was invoked but never defined (see the help page).
  5. ^ Cite error: The named reference Drury and Wallington, 1980 was invoked but never defined (see the help page).
  6. ^ Cite error: The named reference Iezzoni, 2018 was invoked but never defined (see the help page).