| Pho4 | |||||||
|---|---|---|---|---|---|---|---|
 Crystallographic structure of yeast Pho4 homodimer (cyan and green) complexed with DNA (brown).[1] | |||||||
| Identifiers | |||||||
| Symbol | Pho4 | ||||||
| Alt. symbols | YFR034C | ||||||
| NCBI gene | 850594 | ||||||
| PDB | 1a0a | ||||||
| RefSeq | NP_116692 | ||||||
| UniProt | P07270 | ||||||
| |||||||
Pho4 is a protein with a basic helix-loop-helix (bHLH) transcription factor. It is found in S. cerevisiae and other yeasts.[2] It functions as a transcription factor to regulate phosphate responsive genes located in yeast cells.[3] The Pho4 protein homodimer is able to do this by binding to DNA sequences containing the bHLH binding site 5'-CACGTG-3'.[4] This sequence is found in the promoters of genes up-regulated in response to phosphate availability such as the PHO5 gene.[5]
- ^ PDB: 1a0a; Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, et al. (August 1997). "Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition". The EMBO Journal. 16 (15): 4689–97. doi:10.1093/emboj/16.15.4689. PMC 1170095. PMID 9303313.
- ^ Berben G, Legrain M, Gilliquet V, Hilger F (1990). "The yeast regulatory gene PHO4 encodes a helix-loop-helix motif". Yeast. 6 (5): 451–4. doi:10.1002/yea.320060510. PMID 2220078. S2CID 40974115.
- ^ Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, et al. (August 1997). "Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition". The EMBO Journal. 16 (15): 4689–97. doi:10.1093/emboj/16.15.4689. PMC 1170095. PMID 9303313.
- ^ Shao D, Creasy CL, Bergman LW (February 1998). "A cysteine residue in helixII of the bHLH domain is essential for homodimerization of the yeast transcription factor Pho4p". Nucleic Acids Research. 26 (3): 710–4. doi:10.1093/nar/26.3.710. PMC 147311. PMID 9443961.
- ^ Ogawa N, Oshima Y (May 1990). "Functional domains of a positive regulatory protein, PHO4, for transcriptional control of the phosphatase regulon in Saccharomyces cerevisiae". Molecular and Cellular Biology. 10 (5): 2224–36. doi:10.1128/mcb.10.5.2224. PMC 360570. PMID 2183025.