| Phosphatidate phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.4 | ||||||||
| CAS no. | 9025-77-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The enzyme phosphatidate phosphatase (PAP, EC 3.1.3.4) is a key regulatory enzyme in lipid metabolism, catalyzing the conversion of phosphatidate to diacylglycerol:[1][2]
- a 1,2-diacylglycerol 3-phosphate + H2O a 1,2-diacyl-sn-glycerol + phosphate
The reverse conversion is catalyzed by the enzyme diacylglycerol kinase, which replaces the hydroxyl group on diacylgylcerol with a phosphate from ATP, generating ADP in the process.
In yeast, the forward direction is Mg2+-dependent, while the reverse process is Ca2+-dependent.[3] PAP1, a cytosolic phosphatidate phosphatase found in the lung, is also -dependent, but PAP2, a six-transmembrane-domain integral protein found in the plasma membrane, is not.[4][5]
- ^ "BRENDA - Information on EC 3.1.3.4 - phosphatidate phosphatase". www.brenda-enzymes.org. Retrieved 2017-03-01.
- ^ Smith SW, Weiss SB, Kennedy EP (October 1957). "The enzymatic dephosphorylation of phosphatidic acids". J. Biol. Chem. 228 (2): 915–22. PMID 13475370.
- ^ Carman, George M.; Han, Gil-Soo (2009-01-30). "Phosphatidic Acid Phosphatase, a Key Enzyme in the Regulation of Lipid Synthesis". The Journal of Biological Chemistry. 284 (5): 2593–2597. doi:10.1074/jbc.R800059200. ISSN 0021-9258. PMC 2631973. PMID 18812320.
- ^ Carman, George M.; Han, Gil-Soo (2006-12-01). "Roles of phosphatidate phosphatase enzymes in lipid metabolism". Trends in Biochemical Sciences. 31 (12): 694–699. doi:10.1016/j.tibs.2006.10.003. ISSN 0968-0004. PMC 1769311. PMID 17079146.
- ^ Nanjundan, Meera; Possmayer, Fred (2003-01-01). "Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase". American Journal of Physiology. Lung Cellular and Molecular Physiology. 284 (1): L1–23. doi:10.1152/ajplung.00029.2002. ISSN 1040-0605. PMID 12471011.