Pugliese is an Italian surname meaning "from Puglia". Notable people with the surname include:
- Aaron Pugliese, American writer
- Antonio Pugliese, professional wrestler (aka Tony Parisi)
- Armando Pugliese (1947–2024), Italian stage director and playwright
- Clemente Pugliese Levi (1855–1936), Italian
- Dina Pugliese (born 1974), Canadian television personality
- Frank Pugliese, American TV writer and artistic director
- Giuseppe Pugliese (born 1983), Italian footballer
- Humbert Pugliese (1884–1955), Australian film exhibitor and producer
- Jim Pugliese (born 1952), American percussionist
- Juan Carlos Pugliese (1915–1994), Argentine politician
- Julia Jones-Pugliese (1909–1993), American national champion fencer and coach
- Marcelo Pugliese (born 1968), Argentine discus thrower.
- Mario Pugliese (born 1996), Italian footballer
- Nick Pugliese (born 1985), professional baseball pitcher
- Oronzo Pugliese (1910–1990), Italian football manager
- Osvaldo Pugliese (1905–1995), Argentine tango musician
- Patri J. Pugliese (1950–2007), American historian of science, dance, and fencing
- Rocco Pugliese, lobbyist in Pennsylvania
- Rose Pugliese, Colorado politician
- Joseph Pugliese (aka Joe Pug; born 1984), American musician