Pulsed-field gel electrophoresis

A microbiologist runs a pulsed-field gel electrophoresis test used in bacterial typing

Pulsed-field gel electrophoresis (PFGE) is a technique used for the separation of large DNA molecules by applying an electric field that periodically changes direction to a gel matrix.[1][2] Unlike standard agarose gel electrophoresis, which can separate DNA fragments of up to 50 kb, PFGE resolves fragments up to 10 Mb.[1] This allows for the direct analysis of genomic DNA.[2]

  1. ^ a b Kaufmann, Mary Elizabeth (1998). "Pulsed-Field Gel Electrophoresis". Molecular Bacteriology. Methods in Molecular Medicine. Vol. 15. pp. 33–50. doi:10.1385/0-89603-498-4:33. ISBN 0-89603-498-4. PMID 21390741.
  2. ^ a b Herschleb, Jill; Ananiev, Gene; Schwartz, David C (March 2007). "Pulsed-field gel electrophoresis". Nature Protocols. 2 (3): 677–684. doi:10.1038/nprot.2007.94. PMID 17406630. S2CID 13265518.