Shotgun proteomics

Shotgun proteomics refers to the use of bottom-up proteomics techniques in identifying proteins in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry.[1][2][3][4][5][6] The name is derived from shotgun sequencing of DNA which is itself named after the rapidly expanding, quasi-random firing pattern of a shotgun. The most common method of shotgun proteomics starts with the proteins in the mixture being digested and the resulting peptides are separated by liquid chromatography. Tandem mass spectrometry is then used to identify the peptides.

Targeted proteomics using SRM and data-independent acquisition methods are often considered alternatives to shotgun proteomics in the field of bottom-up proteomics. While shotgun proteomics uses data-dependent selection of precursor ions to generate fragment ion scans, the aforementioned methods use a deterministic method for acquisition of fragment ion scans.

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  4. ^ Hu L, Ye M, Jiang X, Feng S, Zou H (August 2007). "Advances in hyphenated analytical techniques for shotgun proteome and peptidome analysis--a review". Analytica Chimica Acta. 598 (2): 193–204. doi:10.1016/j.aca.2007.07.046. PMID 17719892.
  5. ^ Fournier ML, Gilmore JM, Martin-Brown SA, Washburn MP (August 2007). "Multidimensional separations-based shotgun proteomics". Chemical Reviews. 107 (8): 3654–86. doi:10.1021/cr068279a. PMID 17649983.
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