Trans-acting siRNA

Trans-acting siRNA (abbreviated "ta-siRNA" or "tasiRNA") are a class of small interfering RNA (siRNA) that repress gene expression through post-transcriptional gene silencing in land plants.[1][2][3] Precursor transcripts from TAS loci are polyadenylated and converted to double-stranded RNA, and are then processed into 21-nucleotide-long RNA duplexes with overhangs.[1] These segments are incorporated into an RNA-induced silencing complex (RISC) and direct the sequence-specific cleavage of target mRNA. Ta-siRNAs are classified as siRNA because they arise from double-stranded RNA (dsRNA).[4]

  1. ^ a b Vazquez F, Vaucheret H, Rajagopalan R, Lepers C, Gasciolli V, Mallory AC, Hilbert JL, Bartel DP, Crété P (October 2004). "Endogenous trans-acting siRNAs regulate the accumulation of Arabidopsis mRNAs". Mol. Cell. 16 (1): 69–79. doi:10.1016/j.molcel.2004.09.028. PMID 15469823.
  2. ^ Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS (October 2004). "SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis". Genes Dev. 18 (19): 2368–79. doi:10.1101/gad.1231804. PMC 522987. PMID 15466488.
  3. ^ Axtell MJ, Jan C, Rajagopalan R, Bartel DP (November 2006). "A two-hit trigger for siRNA biogenesis in plants". Cell. 127 (3): 565–77. doi:10.1016/j.cell.2006.09.032. PMID 17081978.
  4. ^ Axtell, Michael J. (29 April 2013). "Classification and Comparison of Small RNAs from Plants". Annual Review of Plant Biology. 64 (1): 137–159. doi:10.1146/annurev-arplant-050312-120043. PMID 23330790.