Transposase

A transposase is any of a class of enzymes capable of binding to the end of a transposon and catalysing its movement to another part of a genome, typically by a cut-and-paste mechanism or a replicative mechanism, in a process known as transposition. The word "transposase" was first coined by the individuals who cloned the enzyme required for transposition of the Tn3 transposon.[1] The existence of transposons was postulated in the late 1940s by Barbara McClintock, who was studying the inheritance of maize, but the actual molecular basis for transposition was described by later groups. McClintock discovered that some segments of chromosomes changed their position, jumping between different loci or from one chromosome to another. The repositioning of these transposons (which coded for color) allowed other genes for pigment to be expressed.[2] Transposition in maize causes changes in color; however, in other organisms, such as bacteria, it can cause antibiotic resistance.[2] Transposition is also important in creating genetic diversity within species and generating adaptability to changing living conditions.[3]

Transposases are classified under EC number EC 2.7.7. Genes encoding transposases are widespread in the genomes of most organisms and are the most abundant genes known.[4] During the course of human evolution, as much as 40% of the human genome has moved around via methods such as transposition of transposons.[2]

  1. ^ Heffron F, McCarthy BJ, Ohtsubo H, Ohtsubo E (December 1979). "DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3". Cell. 18 (4): 1153–63. doi:10.1016/0092-8674(79)90228-9. PMID 391406. S2CID 17775137.
  2. ^ a b c Goodsell D (December 2006). "Transposase". Molecule of the Month. Protein Data Bank.
  3. ^ Reznikoff WS (March 2003). "Tn5 as a model for understanding DNA transposition". Molecular Microbiology. 47 (5): 1199–206. doi:10.1046/j.1365-2958.2003.03382.x. PMID 12603728.
  4. ^ Aziz, R.K., M. Breitbart and R.A. Edwards (2010). Transposases are the most abundant, most ubiquitous genes in nature. Nucleic Acids Research 38(13): 4207–4217.Aziz RK, Breitbart M, Edwards RA (July 2010). "Transposases are the most abundant, most ubiquitous genes in nature". Nucleic Acids Research. 38 (13): 4207–17. doi:10.1093/nar/gkq140. PMC 2910039. PMID 20215432.