Names | |
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IUPAC name
(3Z,3'Z)-3,3'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1Z)hydrazin-2-yl-1-ylidene]bis(5-amino-4-oxo-3,4-dihydronaphthalene-2,7-disulfonic acid)
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Identifiers | |
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3D model (JSmol)
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ChEBI | |
ChEMBL | |
ChemSpider | |
ECHA InfoCard | 100.000.715 |
KEGG | |
PubChem CID
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UNII | |
CompTox Dashboard (EPA)
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Properties | |
C34H24N6Na4O14S4 | |
Molar mass | 872.88 |
Appearance | deep blue in aqueous solution[1] |
Melting point | > 300 °C (572 °F; 573 K) |
10mg/ml [2] | |
Solubility | 20 mg/mL in methyl Cellosolve, and 0.6 mg/mL in ethanol |
Pharmacology | |
S01KX02 (WHO) | |
Hazards | |
Lethal dose or concentration (LD, LC): | |
LD50 (median dose)
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6200 mg/kg (oral, rat) |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Trypan blue is an azo dye. It is a direct dye for cotton textiles.[3] In biosciences, it is used as a vital stain to selectively colour dead tissues or cells blue.
Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell. Hence, dead cells appear as a distinctive blue colour under a microscope. Since live cells are excluded from staining, this staining method is also described as a dye exclusion method.