Tupi may refer to:
- Tupi people of Brazil
- Tupi or Tupian languages, spoken in South America
- Tupi oil field off the coast of Brazil
- Tupi Paulista, a Brazilian municipality
- Tupi, South Cotabato, a Philippine municipality
- Tupi class, a Brazilian Navy version of the Type 209 submarine
- Tupi Football Club, a Brazilian football (soccer) club
- Brazil national rugby union team, nicknamed Os Tupis
- Rede Tupi, the first Brazilian TV network (1950–1980)
- The tupi blackberry, a Mexican hybrid of the Comanche and Uruguai varieties
- Tupi (software), free and open source 2D animation software
- Tupí fermented cheese