The genome is non-segmented negative-sense RNA (nsnsRNA).
The gene order is 3′ – leader – NP – VP35 – VP40 – GP/sGP – VP30 – VP24 – L – trailer – 5′
- NP
- VP35
- VP40
- GP/sGP
- VP30
- VP24
- L - "Large" polymerase - complex with VP35 constitutes RNA-dependent RNA polymerase (RdRp)
-
- Determined Nucletoide sequence of L encoding gene and 5' end of RNA.
- RNA has some folded structure, with self-complementary regions on 5' end, and 3' is complemetary to 5'.
- L protein has conserved amino acid sequences with other nsnsRNA viruses
- Conserved functionality is RNA-dependent RNA polymerase (RdRp)
- VSV L protein is catalyst for 5'-end capping
- VSV mRNA is capped with GDP instead of GTP
- VSV L caps mRNA via "RNA:GDP polyribonucleotidyltransferase activity, in which a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate"
|
NP |
VP35 |
VP40 |
GP/sGP |
VP30 |
VP24 |
L
|
| NP |
|
nucleocapsid |
|
|
nucleocapsid |
nucleocapsid |
|
| VP35 |
|
|
|
|
RdRp |
|
RdRp
|
| VP40 |
|
|
|
|
|
|
|
| GP/sGP |
|
|
|
|
|
|
|
| VP30 |
|
|
|
|
|
nucleocapsid |
RdRp
|
| VP24 |
|
|
|
|
|
|
|
| L |
|
|
|
|
|
|
|
| EBOV dsRNA |
|
X |
|
|
|
|
|
| EBOV mRNA |
|
|
|
|
|
|
capping 5' with GDP
|
| NPC-1 |
|
|
|
entry |
|
|
|