Content | |
---|---|
Description | Structure Validation Server |
Contact | |
Research center | University of Alberta and The Metabolomics Innovation Centre |
Laboratory | Dr. David Wishart |
Primary citation | [1] |
Access | |
Data format | Data Input: PDB coordinate file(s); Data Output: Text and graphical data on protein structure features and quality |
Website | http://vadar.wishartlab.com/ |
Miscellaneous | |
Data release frequency | Every 1-2 years with periodic corrections and updates |
Curation policy | Manually curated |
Volume, Area, Dihedral Angle Reporter (VADAR) is a freely available protein structure validation web server that was developed as a collaboration between Dr. Brian Sykes and Dr. David Wishart at the University of Alberta.[1] VADAR consists of over 15 different algorithms and programs for assessing and validating peptide and protein structures from their PDB coordinate data. VADAR is capable of determining secondary structure (using three different algorithms), identifying and classifying six different types of beta turns, determining and calculating the strength of C=O -- N-H hydrogen bonds, calculating residue-specific accessible surface areas (ASA), calculating residue volumes, determining backbone and side chain torsion angles (phi, psi, omega and chi angles), assessing local structure quality (through numerous quality indices), evaluating global structure quality, and identifying residue "outliers" (residues with unusual structural features). The results have been validated through extensive comparison to published data and careful visual inspection. VADAR produces both text and graphical output with most of the quantitative data presented in easily viewed tables. In particular, VADAR's output is presented in a vertical, tabular format with most of the sequence data, residue numbering and any other calculated property or feature presented from top to bottom, rather than from left to right.