The Whitney and Opeongo Railway (W&OR) was a logging railway in Ontario, Canada. It ran from Opeongo Lake to Whitney, where it connected to the Canada Atlantic Railway (CAR), running a total distance of about 14 miles (23 km). It opened in 1902 and closed in the 1920s with the end of major logging operations in the area.
The southern end of the former route was taken over as the basis of Ontario Highway 60 for a section west of Whitney in Algonquin Park, while the northern end to Opeongo Lake is now used as a park access road. Only a short section of the original railbed is no longer used, abandoned when the highway was constructed further west.