12 o'clock usually refers the time as shown on a 12-hour clock, either noon - 12 o'clock at daytime - or midnight - 12 o'clock at nighttime.
12 o'clock may also refer to:
- 12-hour clock, a time convention in which the 24 hours of the day are divided into two periods
- Noon, 12 o'clock in the daytime, as opposed to midnight
- Midnight, the transition time from one day to the next
- The corresponding clock position, straight ahead or directly above
- 12 O'Clock (film), a 1958 Bollywood film
- 12 'O' Clock (film) a 2020 Bollywood film
- 12 O'Clock (rapper), a Wu-Tang Clan affiliate and MC
- 12:00 (Loona EP), 2020
- Twelve O'Clock Point, a community in Quinte West, Ontario, Canada
- "12 O'Clock" (in two parts), a song by Vangelis from Heaven and Hell