| 1940 Jacksonville State Eagle Owls football | |
|---|---|
| Conference | Alabama Intercollegiate Conference |
| Record | 3–5 (3–2 AIC) |
| Head coach |
|
| Home stadium | College Field |
The 1940 Jacksonville State Eagle Owls football team represented Jacksonville State Teachers College (now known as Jacksonville State University) as a member of the Alabama Intercollegiate Conference (AIC) during the 1940 college football season. Led by first-year head coach Osmo Smith, the Eagle Owls compiled an overall record of 3–5 with a mark of 3–2 in conference play.