Elections to Preston Borough Council were held on 7 May 2000. One third of the council was up for election and the council stayed under no overall control.
After the election, the composition of the council was:
| Party | Seats | ± | |
|---|---|---|---|
| Labour | 24 | -4 | |
| Conservative | 17 | +4 | |
| Liberal Democrat | 12 | +1 | |
| Independent | 4 | -1 | |